Hotstart Taq ДНК-полимераза Горячий старт ПЦР 18000U

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Характеристики

 

Optimus^TM Hotstart Taq DNA Polymerase

For research use only

 

 

Cat No. P1041   250U

         P1042   1000U

    P1043   3000U

         P1044   18,000U

 

Components  

Component	P1041	P1042	P1043	P1044
OptimusTM Hotstart Taq DNA Polymerase (5U/ul)	50μl	200μl	200μlx3	100μlx36
10x Hotstart Buffer(Mg2+ Plus)	1.25ml	1.25mlx2	1.25mlx6	1.25mlx36
6xLoading Buffer	1ml	1ml	1ml

 

Storage

2 years at -20°C

 

Description

Optimus^TM Hotstart Taq DNA polymerase is a hot-start polymerase with chemical modification, which brings higher specificity by reducing non-specific products as the enzyme activity is temperature-dependent and is inhibited at room temperature. The amplification length and speed can reach to 5 kb (simple template) and 0.5 kb/min separately. Hotstart Taq has 5’-3’ polymerase activity, but no 3’-5’ exonuclease activity. The products of Taq plus have overhanged dA at 3’-end.

Optimus^TM Hotstart Taq DNA polymerase has zero animal source pollution by being produced with advanced chemical modification. And it is much more stable than antibody-modified hot-start polymerase. Its efficiency is higher than most chemical-modified polymerase and the initial-denaturation time can be reduced to 3 minutes.

Optimus^TM Hotstart Taq DNA polymerase is an innovative and useful product. .

 

 

Unit Definition

One enzyme unit (U) refers to the amount of enzyme needed for intaking 10 nmol nucleotides when using activated salmon sperm DNA as template/primer, at 72 ℃, in 30 minutes

 

Quality control

The absence of endonuclease or exonuclease is confirmed by appropriate quality tests. PCR detects no host residual DNA, and it can effectively amplify the single-copy gene of human genome. There is no significant change about the amplification activity after one week at room temperature.

 

Storage Buffer

200 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Tween 20, 50% Glycerol

0.5% Triton X -100

 

10X Hotstart Buffer with Mg²⁺ 

50 mM KCl, 100 mM Tris-HCl, 200 mM NH₄Cl, 20 mM MgCl₂

 

 

 

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Dongsheng Biotech PCR Reagents are looking for distributors and providing OEM service

Dongsheng Biotech offers different series of products to help you achieve PCR success. For enzymes, our Taq Polymerase, HS Taq DNA Polymerase, FS Taq DNA Polymerase, Pfu DNA Polymerase and Fusion Pfu DNA Polymerase provide high fidelity, efficient, sensitivity PCR performance. We also have Long Taq DNA Polymerase for long PCR performance.