Apramycin сульфат, CAS 65710-07-8
US $26.00-$28.00
Высококачественный реагент для исследований гигромицин B по хорошей цене CAS
- Категория: Химические реагенты >>>
- Поставщик: Nanjing,Duly,Biotech,Co.,Ltd.
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Трекер стоимости
| Месяц | Минимальная цена | Макс. стоимость |
|---|---|---|
| Sep-19-2025 | 83.80 $* | 87.7 $* |
| Aug-19-2025 | 83.11 $* | 87.84 $* |
| Jul-19-2025 | 69.13 $* | 72.14 $* |
| Jun-19-2025 | 81.98 $* | 85.36 $* |
| May-19-2025 | 71.76 $* | 75.71 $* |
| Apr-19-2025 | 80.48 $* | 84.85 $* |
| Mar-19-2025 | 79.10 $* | 83.98 $* |
| Feb-19-2025 | 78.15 $* | 82.90 $* |
| Jan-19-2025 | 78.9 $* | 82.24 $* |
Характеристики
| Grouping | Antibiotics |
| Keywords | Hygromycin B |
| Brand | NJDULY |
| Model | A0026 |
| Place of Origin | Nanjing, China (Mainland) |
| Payment | T/T |
| Port | Shanghai |
| Supply Capacity | 100 per piece/Month |
| Packing | 1 g/piece |
| Other packaging | please consult customer service |
| To Find Out More, Click Here | |
Hygromycin B
CAS: 31282-04-9
Molecular formula: C20H37N3O13
Molecular weight: 527.52
Purity: >90%
Chemical properties: white to off-white powder, soluble in water, methanol and ethanol.
Uses: biochemical research
Mechanism of action: Hygromycin B is an aminoglycoside antibiotic produced by the metabolism of Streptomyces hygroscopicus, which kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. Hygromycin B inhibits protein synthesis by interfering with 70S ribosome translocation and inducing misreading of mRNA templates. It is currently used to screen and maintain cultured prokaryotic or eukaryotic cells containing a hygromycin resistance gene.
Resistance gene:
Resistance to hygromycin B is derived from a gene encoding hygromycin B phosphotransferase (Hph). Two sources have been discovered so far:
♦ Hph resistance gene produced by Streptomyces hygroscopicus;
♦ Escherichia coli, the Hph resistance gene carried by the plasmid in Klebsiella pneumoniae (commonly used);
Biological applications:
1) screening and maintaining cultured prokaryotic or eukaryotic cells (plant cells and mammalian cells) stably transfected with Hph+ vector;
2) Due to the difference in mode of action, it is often used in combination with G418 (GeneticinTM), ZeocinTM and blasticidin S to screen cell lines stably transfected with two different vectors;
CAS: 31282-04-9
Molecular formula: C20H37N3O13
Molecular weight: 527.52
Purity: >90%
Chemical properties: white to off-white powder, soluble in water, methanol and ethanol.
Uses: biochemical research
Mechanism of action: Hygromycin B is an aminoglycoside antibiotic produced by the metabolism of Streptomyces hygroscopicus, which kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. Hygromycin B inhibits protein synthesis by interfering with 70S ribosome translocation and inducing misreading of mRNA templates. It is currently used to screen and maintain cultured prokaryotic or eukaryotic cells containing a hygromycin resistance gene.
Resistance gene:
Resistance to hygromycin B is derived from a gene encoding hygromycin B phosphotransferase (Hph). Two sources have been discovered so far:
♦ Hph resistance gene produced by Streptomyces hygroscopicus;
♦ Escherichia coli, the Hph resistance gene carried by the plasmid in Klebsiella pneumoniae (commonly used);
Biological applications:
1) screening and maintaining cultured prokaryotic or eukaryotic cells (plant cells and mammalian cells) stably transfected with Hph+ vector;
2) Due to the difference in mode of action, it is often used in combination with G418 (GeneticinTM), ZeocinTM and blasticidin S to screen cell lines stably transfected with two different vectors;
| Antibiotic action mechanism and resistance of double resistant stable transfected cells | |||
| antibiotic | Resistance mechanism | Resistance gene | Mammal screening concentration |
| Hygromycin B | Interference with 70S ribosomal translocation and induction of misreading of mRNA templates | Hph | 200~500μg/mL |
| G418 | Interfering with 80S ribosome function and inhibiting protein synthesis | Tn5/Tn601 | 100~1000μg/mL |
| Zeocin | Incorporation or cleavage of DNA causes cell death | Sh ble | 50~100μg/mL |
| blasticidin S | Inhibition of peptide bond formation on ribosomes | Bsd/BSD | 3~50μg/mL |
3) antiviral agents, because hygromycin B selectively permeates into cells that enhance permeability due to viral infection, and has the effect of inhibiting translation;
4) adding animal feed as an anthelmintic;
Application concentration:
The working concentration of hygromycin B used to screen stable strains varies depending on cell type, medium, growth conditions, and cellular metabolic rate. The recommended concentration is 50-1000 μg/mL, and the optimal concentration is determined by the kill curve.
Mammalian cells · 200-500 μg / mL; bacteria / plant cells · 100-300 μg / mL; fungi · 300-1000 μg / mL;
Product use:
Use one: kill curve to determine the best kill concentration (for reference only, depending on the personal detection system)
(1) Add untransfected cells to a tissue culture grade 96-well plate at a cell density of about 50-200 cells/well; after the cells are attached, add different concentrations to each well (eg, 50-1000 μg/mL, at least 5 200 μL fresh medium of hygromycin B;
(2) Incubate the cells for 10-14 days at 37 ° C in a 5% CO 2 incubator;
(3) After 5-7 days of culture, replace the new culture medium, and the culture solution contains the corresponding concentration of hygromycin B;
(4) After 10-14 days, cell viability is evaluated using a cell proliferation method such as MTT, CCK-8, etc. The toxic effect can also be determined by measuring the number of cell clones or the percentage confluence. The minimum concentration that is capable of killing all cells in 10-14 days is generally selected as the optimal screening concentration.
Use two: screening stable transfected cells
(1) For adherent cells, directly absorb the transfected cell culture medium in a 60 mm culture dish, then add 5-6 ml of fresh medium containing hygromycin B; for suspension cells, sterile conditions 250 x g, centrifuge for 10 min to remove The old transfected cell culture medium is then suspended in about 5 ml of fresh medium containing hygromycin B;
(2) After 5-7 days, replace the fresh medium containing hygromycin B as above;
(3) re-incubate the cells for 5-7 days;
(4) After 10-14 days of incubation, the culture system contained only viable cells capable of expressing the hygromycin B resistance phenotype. Therefore, after the screening, as in step 1, the fresh medium containing no hygromycin B was replaced.![2]()
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4) adding animal feed as an anthelmintic;
Application concentration:
The working concentration of hygromycin B used to screen stable strains varies depending on cell type, medium, growth conditions, and cellular metabolic rate. The recommended concentration is 50-1000 μg/mL, and the optimal concentration is determined by the kill curve.
Mammalian cells · 200-500 μg / mL; bacteria / plant cells · 100-300 μg / mL; fungi · 300-1000 μg / mL;
Product use:
Use one: kill curve to determine the best kill concentration (for reference only, depending on the personal detection system)
(1) Add untransfected cells to a tissue culture grade 96-well plate at a cell density of about 50-200 cells/well; after the cells are attached, add different concentrations to each well (eg, 50-1000 μg/mL, at least 5 200 μL fresh medium of hygromycin B;
(2) Incubate the cells for 10-14 days at 37 ° C in a 5% CO 2 incubator;
(3) After 5-7 days of culture, replace the new culture medium, and the culture solution contains the corresponding concentration of hygromycin B;
(4) After 10-14 days, cell viability is evaluated using a cell proliferation method such as MTT, CCK-8, etc. The toxic effect can also be determined by measuring the number of cell clones or the percentage confluence. The minimum concentration that is capable of killing all cells in 10-14 days is generally selected as the optimal screening concentration.
Use two: screening stable transfected cells
(1) For adherent cells, directly absorb the transfected cell culture medium in a 60 mm culture dish, then add 5-6 ml of fresh medium containing hygromycin B; for suspension cells, sterile conditions 250 x g, centrifuge for 10 min to remove The old transfected cell culture medium is then suspended in about 5 ml of fresh medium containing hygromycin B;
(2) After 5-7 days, replace the fresh medium containing hygromycin B as above;
(3) re-incubate the cells for 5-7 days;
(4) After 10-14 days of incubation, the culture system contained only viable cells capable of expressing the hygromycin B resistance phenotype. Therefore, after the screening, as in step 1, the fresh medium containing no hygromycin B was replaced.
Company Information
Nanjing Duly Biotech Co.,Ltd
has been established for 11 years. It is mainly engaded in biological and chemical reagents,including enzyme reagents, proteins, nucleic acids, pigments, amino acids, vitamins,carbohydrates, buffers, surfactants,culture media and Separation of materials and kits.There are more than 10,000 products in total.
Our company’s aim is to provide customers with the best quality products and the most intimate service.Personnel has been trained; every batch of material is checked; each procedure guarantees the quality; every bag of our products is top grade;service is thoughtful all the time.The quality, colors and forms of our products have reached a high level in related chemical industry, which relies on characteristic and advanced processing technology and it has gained a high and reliable reputation from customers at both quality and services.
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Answer: It is mainly engaded in biological and chemical reagents,including enzyme reagents, proteins, antibiotics, plant hormones, nucleic acids, pigments, amino acids, vitamins, carbohydrates, buffers, surfactants, culture media and Separation of materials and kits.
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Answer: Mainly do small packaging reagents, large packaging also do, specific can ask customer service personnel.
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