Кассета для быстрого тестирования энтеровируса 71 (EV71)-антитела

Описание и отзывы

Трекер стоимости

Характеристики

rapid test cassette for enterovirus 71 (EV71)-IgM antibody

Intended Use

rapid test cassette for enterovirus 71 (EV71)-IgM antibody is used to qualitatively detect the EV71- IgM antibody in human blood, serum or plasma samples. It is a diagnosis reagent of EV early infection in clinical diagnosis. Enterovirus type 71 is one of the Picornaviridae virus (enterovirus, EV) genus, group A. It has obvious properties of dermatotropic and neurotropic. Being infected by EV71 can cause hand-foot-mouth disease, Herpes pharyngitis, Aseptic meningitis and encephalitis, encephalitis and the paralysis of poliomyelitis and recessive infection in more cases. Human being is the only known natural host of EV71. The EV71 is mainly infected by the fecal - oral transmission. EV71 has a world-wide distribution. Its epidemic season is summer and autumn, but it can infect during all year. Human is the generally susceptible of EV71, while the infants and young children are more dangerous.

Principles of the Tests

rapid test cassette for enterovirus 71 (EV71)-IgM antibody  is applying the technology of colloidal gold immunochromatography to qualitative test the EV71- IgM antibody of whole blood, serum or plasma samples. The EV71-IgM antibodies in positive sample can combine with colloidal gold labeled mouse anti human IgM immobilized on conjugate pad to form immune complex, and move along the membrane strip by chromatography. Some of the complex is caught by recombinant EV71-Ag pre-coated on T line to form "gold labeled mouse anti human IgM- EV71 IgM Ab- EV71 Ag" and develop color. The remaining free complex will be caught by the goat anti-mouse IgG antibody coated on the C line to develop color. As for negative samples, in which have no EV71- IgM antibodies, cannot form immune complexes and develop color on the T line, and colorate on C line only.

 

Main Constituent

1. 1.        Test Card ( Coating the recombinant EV71 VP1-VP3 antigen on T-line, the goat anti-mouse IgG antibody on C-line. 25 test cards/box
2. 2.        The sample diluent: 1 bottle (5 ml)
3. 3.        Product instruction: 1

 

Storage conditions and the period of validity

 

Store at 4~30°C, dry places for 12 months. Use test card within 1 hour once open to atmosphere when the humidity is below 60%, or use it immediately if the humidity is higher.

Sample Request

1. 1.        Serum samples were collected from the venous blood by conventional method. Plasma sample: add 100ul heparin solution (1%) to 5 ~ 10ml blood; or sodium citrate solution (3.8%) to plasma according to the proportion of 1:9; or EDTA solution (15%) 0.04ml to 5ml plasma.
2. 2.        Serum and plasma samples can be stored at 4 °C if tests will be done within 5 days, otherwise stored at -20 °C. No more than 3 times of freeze-thaw.
3. 3.        Whole blood samples should be stored at 2-8°C and tested within 3 days, and cannot be frozen.
4. 4.        The test result is invalid for hemolysis sample