2.lnjection
The lnjection of oxytocin can be divided into one injection method and two lnjection method, and there are even three injections used in broodstock to induce labor. The broodstock matures well, and the one lnjection method is usually used when the water temperature is appropriate, but generally the effect of the second lnjection method is better than the one lnjection method. Its spawning rate, egg production and fertilization rate are higher, and the broodstock's estrus time is more consistent. It is especially suitable for inducing spawning at an early stage or when the maturity of the broodstock is not enough, because the first injection has the effect of ripening. The second lnjection method only injects a small amount of oxytocin for the first time, and then injects the entire remaining dose a few hours later. The interval between two lnjections is 6~24 hours. Generally speaking, when the water temperature is low or the broodstock is not mature enough, the interval should be longer, otherwise it should be shorter. Before lnjection, use fish clips to extract the broodstock and weigh, and then calculate the actual dose to be injected. During the lnjection, one person takes the fish clip and makes the fish lie on its side, exposing the lnjection site, and the other person injects it. Use 5ml, 10ml or continuous veterinary syringes for syringes, with needles of No. 6-8, and need to be boiled and sterilized before use. There is a scaly depression at the base of the pectoral fin of the pectoral lnjection at the lnjection site. The height of the lnjection is to pierce the needle toward the front of the fish body at an angle of 45 to 60 degrees to the body axis. The depth is generally about 1 cm. It should not be too deep, otherwise it will hurt. And internal organs. Inject into the base of the pelvic fin by intraperitoneal lnjection, the injection angle is about 30~45 degrees, and the depth is 1~2 cm. Intramuscular lnjection is generally performed at the full muscle under the dorsal fin. A needle is used to pierce the muscle 1~2 cm forward along the scale for injection. After the lnjection is completed, quickly pull out the needle, and disinfect the lnjection port with iodine to prevent lnfection. If the fish is struggling and agitated during the lnjection, the needle should be pulled out quickly to avoid injury to the fish. In terms of production, in order to control the fish to spawn in the morning and facilitate work, the lnjection time should be determined according to the weather, water temperature and the effect time. Generally, one-time lnjections are carried out in the afternoon, and the eggs are laid in the early morning of the next day. The second lnjection method is based on the time of the second injection. Generally, the first injection is performed at around 9 am, and the second injection is performed at 6 to 8 o'clock in the same day. Areas with large daily temperature differences can be moved back for 1 to 3 hours so that the water temperature is higher during spawning. The time required for the broodstock to start estrus after the injection of the oxytocin is called the effect time. The effect time varies from a few hours to more than 20 hours depending on the situation. The length of the effect time is mainly determined by the water temperature, the effect time is short when the water temperature is high, and vice versa. Generally, the effect time of the second lnjection is shorter than that of the first injection. The relationship between the water temperature and the number of lnjections and the effect time. Generally, the effect time of the pituitary gland is shorter than that of chorionic hormone, and chorionic hormone is shorter than its analogs. Usually bighead carp has the longest effect time, grass carp has the shortest effect time, and silver carp and herring have similar effect time.