Набор ДНК/РНК HiPure FFPE (ivd) IVD5116 коизоляционная ДНК и РНК из образца
- Категория: Chemical Reagent Products >>>
- Поставщик: Guangzhou,Magen,Biotechnology,Co.,Ltd.
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Описание и отзывы
Трекер стоимости
| Месяц | Минимальная цена | Макс. стоимость |
|---|---|---|
| Sep-17-2025 | 0.54 $* | 0.55 $* |
| Aug-17-2025 | 0.57 $* | 0.23 $* |
| Jul-17-2025 | 0.38 $* | 0.28 $* |
| Jun-17-2025 | 0.65 $* | 0.56 $* |
| May-17-2025 | 0.42 $* | 0.41 $* |
| Apr-17-2025 | 0.61 $* | 0.13 $* |
| Mar-17-2025 | 0.59 $* | 0.23 $* |
| Feb-17-2025 | 0.68 $* | 0.32 $* |
| Jan-17-2025 | 0.55 $* | 0.14 $* |
Характеристики
Overview
Product Name: HiPure FFPE DNA/RNA Kit (ivd)
Product Use Case: co-Isolation DNA and RNA from a single FFPE Tissue sample
Packaging Size: 50 Preps/box
Storage：Room temperature
Shelf Life: 18 months
Product Use Case: co-Isolation DNA and RNA from a single FFPE Tissue sample
Packaging Size: 50 Preps/box
Storage：Room temperature
Shelf Life: 18 months
Introduction
This product is designed for extraction of both RNA and DNA from FFPE samples. The purified DNA/RNA can be used for clinical in vitro testing. This product is based on silica memrane purification method. The sample is lysed and digested under the lysate solution and proteinase K and DNA and RNA are released. After binding conditions adjustment, DNA or RNA will be adsorbed on the column membrane, while impurities such as protein will not be adsorbed and removed. The filter membrane with adsorbed nucleic acid is washed with washing solution to remove protein and impurities, and washed with ethanol to remove salt; DNA/RNA is eluted by low-salt buufer or purified water finally.
Procedure
This product is based on the purification method of high binding magnetic particles. DNA and RNA are released sequentially by differential solubilization of the same precious FFPE sample. After solubilization, both nucleic acids are treated separately to remove formaldehyde cross-links and transfer to an adsorption filter column. DNA/RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA / RNA was finally eluted with low-salt buffer (10 Mm Tris, pH 8.0).
Specifications
Main function | co-Isolation DNA and RNA from a single FFPE Tissue sample |
Applications | RT-PCR, Northern blot, poly-A+, PCR, Sanger Sequencing, cDNA synthesis |
Binding medium | Spin column with Glass fiber membrane |
Operation Method | Centrifuge or Vacuum |
Sample type | FFPE section, FFPE embedded tissue |
Sample Volume | - |
DNA and RNA yield | DNA: 2-20ug; RNA:5-50ug |
Column Binding Capacity | 100 ug |
Column holding Volume Capacity | 800ul |
Minimum Elution Volume | 30 ul |
Extraction time | less than 50 minutes |
Operation | manual |
Kit Contents
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