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Grouping	Other reagents
Keywords	DNS Reagent
Brand	NJDULY
Place of Origin	Nanjing, China (Mainland)
Payment	T/T
Port	Shanghai
Supply Capacity	100  Piece/per Month
Packing	100ml/piece
Other packaging	please consult customer service
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Product introduction
DNS (dinitrosalicylic acid) method is based on the principle that under alkaline conditions, dinitrosalicylic acid (DNS) reacts with reducing sugar to form 3-amino-5-nitrosalicylic acid. The product is brownish red under boiling conditions, and the color depth is proportional to the reducing sugar content in a certain concentration range. The content of reducing sugar is determined by colorimetry. Because the color development is only related to the amount of free reducing groups of carbohydrates, but has no selectivity to the types of reducing sugars, DNS method is suitable for the hydrolysis of polysaccharides (such as cellulose, hemicellulose and starch).
Operation steps (for reference only)
1. The extraction of reducing sugar was as follows
2. Hydrolysis and extraction of total sugar
3. Preparation of glucose standard curve: take clean centrifuge tube or test tube, operate according to the following table, set zero with No.0, detect the absorbance at 540nm, draw the standard curve with absorbance value as ordinate and each standard concentration (mg / ml) as abscissa.

Adding substances(ml)	0	1	2	3	4	5
Glustandard(1mg/ml)	0	0.2	0.4	0.6	0.8	1
distilled water	1	0.8	0.6	0.4	0.2	0
DNSreagent	2	2	2	2	2	2
In a boiling water bath5Minutes later, remove and cool to room temperature with running water
distilled water	9	9	9	9	9	9
Equivalent to the amount of grape sugar	0	0.2	0.4	0.6	0.8	1

 

 4. Determination of reducing sugar: take 1ml of prepared reducing sugar extraction solution or total sugar hydrolysate solution, add 2ml DNS reagent respectively. Other operations are the same as those of standard curve, and the absorbance of each tube is measured at 540nm.
Calculation:
Percentage of reducing sugar:
Reducing sugar content (%) = (C × VT) / (m × VS) × 100
The total sugar content was as follows
Total sugar content (%) = (C × VT) / (m × VS) × 100 × 10 × 0.9
Precautions:
1. The above-mentioned low-temperature reagents should avoid repeated freezing and thawing, so as to avoid failure or decrease of efficiency. A kind of
2. If the sample can not be stored in time for 3 days, the sample should be stored in time.
3. If the concentration of reducing sugar in the sample is too high, dilute it with distilled water and retest, and multiply the result with dilution multiple. A kind of
4. The calculation formula of total sugar is used when there are few interference impurities and the content of reducing sugar is less than that of total sugar. The purpose of x 0.9 is to deduct the water consumption during hydrolysis from the determined total sugar to monosaccharide.