Набор ДНК крови TIANamp для экстракции геномистой очистки нуклеиновой

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 TIANamp Blood DNA Kit For Genomic DNA Extraction For Nucleic Acid Purification

 

   The TIANamp Blood DNA Kit uses spin columns which can specifically bind to DNA, and provides unique buffer system for effective blood gDNA extraction. The spin column is made of new type silica membrane which can bind DNA efficiently and specifically. It can maximally remove contaminant proteins and other organic compounds in cells. Genomic DNA isolated by the kit is stable with good integrity and purity.

Features:

1.Wide application: The kit could be used to extract gDNA from antico agulant blood (EDTA, heparin, etc.), buffy coat and blood clots directly.

2. High quality: With the unique lysis buffer system, the purified DNA with high concentration, purity and good integrality could satisfy the demands of chip hybridization and high-throughput sequencing.

3.Rapid and non-toxic: The kit uses silica membrane adsorption technology and does not need phenol and chloroform. The whole extraction process could be completed within 1 hour.

Contents	200 preps
Buffer CL	250 ml
Buffer GS	50 ml
Buffer GB	50 ml
Buffer BD	80 ml
Buffer GDB	120 ml
Buffer PWB	50 ml
Buffer TB	60 ml
Proteinase K	4×1 ml
Spin columns CG2	200
Collection tubes (2 ml)	200
Centrifuge tubes (1.5 ml)	200

Important Notes:

1. To remove RNA residues, RNase A solution (100 mg/ml) needs to be self-prepared.

2. This product has been registered as the class I in vitro diagnostic reagent by Beijing FDA. Please contact TIANGEN if further information is needed.

 

Genomic DNA were extracted from blood samples using TIANamp Blood DNA Kit.

M: TIANGEN Marker D15000.

Samples: 200 μl human anticoagulation blood with EDTA, EDTA/NaF, heparin and sodium citrate; buffy coat from 2 ml blood; 10 μl chicken blood and 200 μl rat blood. For buffy coat, 2 μl of 100 μl eluates were loaded; for the others, 4 μl of 100 μl eluates were loaded per lane.

Experimental results: TIANamp Blood DNA Kit can be widely applied in the high-quality DNA purification from human anticoagulants with different treatments as well as the blood from various species.

Comparison of the genomic DNA extracted from 200 μl human anticoagulation with EDTA using TIANamp Blood DNA Kit and similar products from other suppliers. M: TIANGEN Marker D15000. 4 μl of 100 μl eluates were loaded per lane.

Experimental results: Compare to other suppliers, TIANamp Blood DNA Kit can obtain higher yield of blood gDNA.