Профессиональный комплект RNAprep Pure Plant Plus для очистки РНК нуклеиновой

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Professional RNAprep Pure Plant Plus Kit For Nucleic Acid Purification

 

   The RNAprep Pure Plant Plus Kit (Polysaccharides & Polyphenolics-rich) is a silicon matrix purification RNA extraction kit developed for multiple plant samples with polysaccharides and polyphenolics. It is supplied with Buffer SL with superb lysis ability. High-purity total RNA without gDNA can be extracted from the fleshes of bananas, watermelons, apples, pears, the tubers of sweet potatoes, potatoes, as well as the leaves of cotton, rose, alfalfa, rice and white pine needles within 1 h.

Features: 

1.Targeted: It is specially formulated for plant samples that are difficult to extract, such as polysaccharides and polyphenolics-rich plants. The process is more optimized, and the results are reliable.

2.Efficient removal of gDNA: High-efficient DNase I is supplied for rapid removal of gDNA on the column.

3.Easy and fast: RNA extraction experiments can be completed in just 1 hour.

4.Safe and low toxicity: no toxic organic reagents such as phenol and chloroform are needed.

 

Contents	50 preps
Buffer SL	30 ml
Buffer RW1	40 ml
Buffer RW	12 ml
RNase-Free ddH2O (Bottled)	15 ml
RNase-Free Spin Columns CR3 set (with 2 ml collection tubes)	50
RNase-Free Filtration Columns CS set (with 2 ml collection tubes)	50
RNase-Free DNase I (1500 U)	1
Buffer RDD	4 ml
RNase-Free ddH2O (Tubular)	1 ml
RNase-Free Centrifuge Tubes (1.5 ml)	50

Storage:

DNase I, Buffer RDD, RNase-Free ddH2O (Tubular) should be stored at 2-8℃

Other reagents could be store at room temperature (15-25℃)

 

Total RNA was extracted from 100 mg fleshes of banana, watermelon, apple and pear, tubers of sweet potato and potato, leaves of cotton, rose, alfalfa, rice and white pine needles respectively using RNAprep Pure Plant Plus Kit. 4-6 μl of 30 μl eluates were loaded per lane.

M: TIANGEN Marker III;

The electrophoresis was conducted at 6 V/cm for 30 min on a 1% agarose.

Results: RNAprep Pure Plant Plus Kit can extract high purity, high yield and good integrity total RNA from the polysaccharides & polyphenolics-rich plant samples.